These AS & A Level Biology 9700 topics consistently produce the lowest scores. Prioritise these in your revision.
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Protein structure — secondary vs tertiary vs quaternary
Hydrogen bonds in secondary structure (between backbone NH and CO groups) confused with those in tertiary structure (between R-groups). Collagen fibres vs collagen molecules repeatedly confused.
Affects: Paper 1, Paper 2
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Respiration — locations, NAD, and oxidative phosphorylation
Glycolysis placed in mitochondria instead of cytoplasm. NAD called an 'electron carrier' instead of 'hydrogen atom carrier'. Matrix wrongly identified as site of oxidative phosphorylation.
Affects: Paper 4
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Sources and sinks in phloem transport
'Many identified storage cells of seeds as sinks. This is true as seeds develop but when seeds start to grow (germinate), the storage cells become sources.' Leaves can be sinks for amino acids.
Affects: Paper 1, Paper 4
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Phospholipid bilayer — counting membrane crossings
'Many thought a molecule of oxygen would pass through 5 or 6 phospholipid layers.' Each membrane = 2 phospholipid layers (bilayer). Students confuse membranes with layers.
Affects: Paper 1
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Hardy-Weinberg equation application
q confused with q². p² + 2pq lumped together when calculating dominant phenotype. Heterozygote frequency (2pq) frequently halved incorrectly.
Affects: Paper 4
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Meiosis stages — chromosome behaviour at each stage
Telophase II products: 'Few candidates recognised that telophase II results in four daughter cells.' Chromatids drawn joined at centromere after anaphase II (should be separated).
Affects: Paper 4
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Immune system — T-cell differentiation and memory cells
'Many thought T-lymphocytes differentiate into T-helper and T-killer cells only at the time of immune response.' They differentiate during maturation in the thymus. Memory CELLS (not antibodies) provide long-lived immunity.
Affects: Paper 2, Paper 4
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Genetic technology — restriction enzymes, recombinant DNA
'Most candidates had difficulty recalling knowledge from 19.1 Principles of genetic technology.' Restriction enzymes cut DNA (not mRNA). Role of ligase poorly known.
Affects: Paper 4